Myd88 Knockout mouse models
Myd88, the mouse ortholog of human MYD88, encodes the universal TIR domain adaptor for every Toll-like receptor except TLR3 and for IL-1R, IL-18R, and IL-33R, using its death domain to nucleate a helical Myddosome with IRAK4 and IRAK1 or IRAK2 that activates TRAF6, TAK1, IKK, and MAPK signaling. Human MYD88 deficiency yields severe pyogenic bacterial infection with otherwise intact immunity, while the recurrent L265P substitution in the TIR domain drives spontaneous Myddosome assembly and constitutive NF-kB in Waldenstrom macroglobulinemia and activated B cell diffuse large B cell lymphoma, cooperating with CD79B in a B cell receptor associated supercomplex. This split biology dictates the allele fork precisely: a conditional null tests requirement for innate sensing, and an L265P knockin, ideally Cd19-Cre or Aicda-Cre restricted and inducible, models the lymphoma. Since germline null phenotypes are strongly microbiota dependent, use littermate controls and defined flora rather than cross-facility comparisons.
Myd88 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Myd88 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Myd88 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Myd88 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Myd88 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Myd88 Knockout mouse models are available?
When Myd88 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Myd88 knockout embryonic lethal in mice?
It depends on background and allele design. Some Myd88 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Myd88 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Myd88?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.