READY TO SHIP!
DEVELOPING
Ndufa12 Conditional Knockout mouse models
Final maturation of complex I depends on Ndufa12, the mouse ortholog of human NDUFA12, which encodes the B17.2 subunit added late to the N module of complex I, capping the peripheral arm once the FMN and iron-sulfur chain are in place, and it must be distinguished from its paralogous assembly chaperone NDUFAF2, formerly B17.2L, which transiently occupies the same site during biogenesis. Human NDUFA12 loss causes Leigh syndrome and optic atrophy with isolated complex I deficiency, and cells lacking it retain a nearly complete but functionally impaired enzyme, indicating a role in the terminal capping step and in stabilizing electron flow toward quinone. This makes a null allele genuinely informative, since the resulting phenotype is a misfinished rather than absent complex, while patient knockins allow allele-specific severity comparisons. The NDUFAF2 relationship is the real caution here, since retained chaperone occupancy can blunt phenotypes. Align allele with endpoints including in-gel activity, NDUFAF2 retention assays, retinal ganglion cell counts, and brainstem lesion progression.
Tissue restricted knockout of Ndufa12 keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells.
What Ndufa12 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Ndufa12 conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Ndufa12-Flox | CKO | CKO | DEVELOPING | CKO 263457 | Inquire |
| These mice carry loxP sites flanking exon2 of Ndufa12 gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Ndufa12 gene. | |||||
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Ndufa12 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Ndufa12 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Ndufa12 Conditional Knockout mouse models are available?
When Ndufa12 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Ndufa12 knockout embryonic lethal in mice?
It depends on background and allele design. Some Ndufa12 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Ndufa12 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Ndufa12?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ITL
Send the Ndufa12 Conditional Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.