Nf1 Knockout mouse models
Neurofibromin, encoded by mouse Nf1 in orthology with human NF1, is a large cytoplasmic protein whose central GAP-related domain accelerates GTP hydrolysis on RAS, making it the principal brake on RAS-RAF-MEK-ERK and, indirectly, on mTORC1 output; flanking regions mediate SPRED1-dependent membrane recruitment and adenylyl cyclase modulation. Germline heterozygous truncations cause neurofibromatosis type 1, and somatic second hits in Schwann cell precursors, astrocytes, or myeloid progenitors generate plexiform neurofibroma, malignant peripheral nerve sheath tumor, optic pathway glioma, and juvenile myelomonocytic leukemia, while biallelic inactivation also recurs in glioblastoma and melanoma. Because germline nulls die near midgestation from cardiac outflow tract defects, conditional nulls are standard, with Dhh-cre or Krox20-cre for Schwann cell lineages and Gfap-cre for glioma, typically on an Nf1 heterozygous background to reproduce haploinsufficient stroma. Match allele class to endpoint: nulls for tumor initiation and MEK inhibitor response, humanized or specific missense knockins for variant interpretation.
Whole body loss of Nf1 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Nf1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Nf1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Nf1-KO | Knockout | KO/CKO mice, disease model mice | embryo cryopreservation | KO 210301 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Nf1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Nf1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Nf1 Knockout mouse models are available?
When Nf1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Nf1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Nf1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Nf1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Nf1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.