Nf2 Conditional Knockout mouse models
Merlin, the product of mouse Nf2 and ortholog of human NF2, is a FERM domain cytoskeletal adaptor that couples cortical actin and adherens junctions to growth control, restraining the Hippo pathway by promoting LATS1 and LATS2 activation and YAP or TAZ phosphorylation, and separately entering the nucleus to inhibit the CRL4-DCAF1 E3 ligase. Biallelic inactivation underlies familial and sporadic schwannoma, meningioma, ependymoma, and a large fraction of malignant pleural mesothelioma, with tumorigenesis proceeding through unrestrained YAP-TEAD transcription rather than kinase hyperactivation. Germline nulls fail at gastrulation because of extraembryonic defects, so conditional nulls are mandatory: P0-cre or Postn-cre for Schwann cells, Wt1-cre for mesothelium, each yielding distinct tumor spectra. Compensation is limited, since the ezrin, radixin, and moesin family shares the FERM fold without recapitulating growth suppression. Choose nulls for tumor initiation and TEAD inhibitor sensitivity, and consider humanized alleles where clinical missense variants must be functionally classified.
Conditional deletion of Nf2 limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Nf2 floxed stock yields usable cohorts.
What Nf2 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Nf2 conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Nf2-Flox | Conditional Knockout | KO/CKO mice, disease model mice | live | CKO 200017 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Nf2 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Nf2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Nf2 Conditional Knockout mouse models are available?
When Nf2 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Nf2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Nf2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Nf2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Nf2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.