Nfkb1 Conditional Knockout mouse — myeloid specific context
Nfkb1, the mouse ortholog of human NFKB1, encodes p105, which is co-translationally and post-translationally processed to the p50 DNA-binding subunit; the precursor contains a Rel homology domain plus a carboxy-terminal ankyrin repeat region that functions as its own inhibitor, sequestering RelA and c-Rel until stimulus-dependent IKK phosphorylation and betaTrCP-mediated degradation of the ankyrin segment release active dimers. Because p50 lacks a transactivation domain, p50 homodimers act as repressors at kappaB sites, so the gene contributes both activating and silencing functions, and p105 additionally scaffolds Map3k8 to link NF-kB and Erk signaling. Homozygous nulls are viable with defective B cell responses, impaired isotype switching, and heightened infection susceptibility, making germline alleles tractable. A nonprocessable p105 knockin separates precursor inhibitor function from p50 output, conditional alleles restrict loss to a lineage, and humanization supports reagent matching. Rela, Relb, c-Rel, and Nfkb2 overlap at target promoters, so compound genetics is often necessary.
A floxed Nfkb1 allele paired with Cre gives spatial control that whole body knockouts cannot offer. People use it to separate developmental roles from adult homeostasis, to model somatic mutations, and to match disease that begins in one organ. For myeloid work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Catalog options
Conditional knockout focuses the experiment on myeloid while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| Nfkb1-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 233083 | Inquire |
Designing a Nfkb1 Conditional Knockout allele
A conditional allele here cannot separate p105 from p50, since floxing the exons encoding the Rel homology domain removes both at once; if precursor function is the question, use a processing mutant instead. What conditional deletion does deliver is compartment assignment, and the expected direction of effect is not always downward. Because p50 homodimers occupy kappaB sites without a transactivation domain and act as repressors, deletion in myeloid cells with Lyz2-Cre often raises rather than lowers inflammatory transcription. Cd79a-Cre or Cd19-Cre addresses the intrinsic B cell defect in class switching, and Vil1-Cre tests epithelial restraint of colitis. Expect loss of the p105 scaffold to destabilize MAP3K8 protein and reduce ERK output, an off axis consequence to be measured rather than assumed absent. Read kappaB occupancy by supershift or ChIP, and cytokine output after lipopolysaccharide.
Pricing and quotes
The Nfkb1 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Nfkb1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with milestones for genotyping, QC, and dispatch.
FAQ
What Nfkb1 Conditional Knockout mouse models are available?
When Nfkb1 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Nfkb1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Nfkb1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for myeloid specific focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. Your note points to myeloid specific, so we start driver selection there.
Do you ship live Nfkb1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Nfkb1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.