Nlgn1 Knockout mouse models
Nlgn1, the mouse ortholog of human NLGN1, encodes neuroligin-1, a postsynaptic cell adhesion molecule with an extracellular cholinesterase-like domain that binds presynaptic neurexins in a calcium-dependent manner and a short cytoplasmic tail whose PDZ-binding motif recruits PSD-95 and whose gephyrin-independent interactions organize the excitatory postsynaptic density. Neuroligin-1 is targeted specifically to glutamatergic synapses by inclusion of splice site B, which biases binding toward beta-neurexins lacking splice site 4, and it preferentially controls NMDA receptor content, so deletion reduces the NMDA to AMPA ratio and impairs long-term potentiation and spatial memory without changing synapse number substantially. The protein is also shed by matrix metalloproteinase 9 in an activity-dependent manner, a step required for plasticity. Conditional nulls with region-restricted Cre drivers assign these effects to defined circuits, while splice site or PDZ motif knockins separate adhesion from scaffolding. Nlgn2 and Nlgn3 occupy distinct synapse classes and only partially buffer loss, so measure receptor composition directly.
Nlgn1 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Nlgn1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Nlgn1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Nlgn1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 2100727 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Nlgn1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Nlgn1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Nlgn1 Knockout mouse models are available?
When Nlgn1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Nlgn1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Nlgn1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Nlgn1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Nlgn1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.