Nodal Knockout mouse models
Nodal, the mouse ortholog of human NODAL, encodes a TGF-beta superfamily ligand unique in requiring an EGF-CFC coreceptor, Cripto or Cryptic, to assemble ActRIIA or ActRIIB with ALK4 and phosphorylate SMAD2 and SMAD3 for transcription with SMAD4, FOXH1, and MIXL1, and whose signaling range is set by convertase processing through Furin and PACE4 and by the antagonists Lefty1, Lefty2, and Cerberus. It drives primitive streak formation, mesendoderm specification, node function, and left-right asymmetry through an autoregulatory asymmetric enhancer, and human variants cause heterotaxy with transposition of the great arteries. Nulls arrest before streak formation, so conditional and hypomorphic alleles, or deletion of the asymmetric enhancer alone, are the only informative routes to later requirements. Gdf1 is not a spare copy but an obligate heterodimer partner for long-range signaling, so compound Gdf1 genetics and precisely timed Cre induction determine whether results are interpretable.
A conventional Nodal knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Nodal Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Nodal knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Nodal-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 190536 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Nodal Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Nodal allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Nodal Knockout mouse models are available?
When Nodal Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Nodal knockout embryonic lethal in mice?
It depends on background and allele design. Some Nodal germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Nodal animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Nodal?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.