What Nodal Knockout mouse models are available?

When Nodal Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.

Is Nodal knockout embryonic lethal in mice?

It depends on background and allele design. Some Nodal germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.

Which Cre driver is best for experiments?

Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.

Do you ship live Nodal animals?

When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.

How do I request a quote for Nodal?

Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.

●1 Model Available

Nodal Knockout mouse model

Name: Nodal-KO
Brand: ingenious targeting laboratory
SKU: KO 190536
Availability: PreOrder

Browse 1 Nodal mouse model including knockout variants from ingenious targeting laboratory. Contact us for availability and fast turnaround.

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Why model Nodal

Nodal, the mouse ortholog of human NODAL, encodes a TGF-beta superfamily ligand unique in requiring an EGF-CFC coreceptor, Cripto or Cryptic, to assemble ActRIIA or ActRIIB with ALK4 and phosphorylate SMAD2 and SMAD3 for transcription with SMAD4, FOXH1, and MIXL1, and whose signaling range is set by convertase processing through Furin and PACE4 and by the antagonists Lefty1, Lefty2, and Cerberus. It drives primitive streak formation, mesendoderm specification, node function, and left-right asymmetry through an autoregulatory asymmetric enhancer, and human variants cause heterotaxy with transposition of the great arteries. Nulls arrest before streak formation, so conditional and hypomorphic alleles, or deletion of the asymmetric enhancer alone, are the only informative routes to later requirements. Gdf1 is not a spare copy but an obligate heterodimer partner for long-range signaling, so compound Gdf1 genetics and precisely timed Cre induction determine whether results are interpretable.

What Nodal mouse models are available?

ingenious targeting laboratory offers 1 distinct Nodal (NODAL) catalog mouse model in the TGF-beta/Activin/Nodal family, featuring conditional knockout and standard knockout. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.

Model availability, allele class options, and quality control standards
Model Availability	Allele Class Options	Quality Control Standards
1 Ready Catalog Line	Conditional Knockout (cKO)	Documented Germline Transmission
Custom Model Generation	Constitutive Knockout (KO)	Strict United States QC Guidelines
Advanced Modifications	Humanized, Knockin, Transgenic	Cryo or Live Dispatch Options

Quality Control: Documented Germline Transmission
Quality Control: Strict United States QC Guidelines
Quality Control: Cryo or Live Dispatch Options

Nodal model types

PathAvailability

Knockout
View catalog
Conditional knockout
View catalog
Knockin
Request a quote
Humanized
Request a quote
Transgenic / overexpression
Request a quote

Prefer the order form? Order. Need an allele that is not listed? Request a quote.

Nodal allele types

Knockout, conditional, knockin, humanized, and related paths for Nodal (human NODAL). Catalog lines ship when inventory exists. Everything else is a generation quote.

Knockout

Conventional / global / constitutive KO

Conditional KO (floxed / loxP)

Inducible conditional KO (CreER / tet)

Tissue specific KO

Specify tissue or Cre driver on quote

Knockout first (tm1a / IKMC)

Convertible floxed allele pathway

Double / compound KO

Multi allele / compound knockout project

Large scale / BAC deletion

BAC scale deletion or targeting

Knockin

Point mutation KI

cDNA KI

Reporter KI (GFP, YFP, RFP, mCherry, tdTomato, lacZ, luciferase)

Tag KI (FLAG, HA, Myc, V5)

Conditional KI (e.g. Rosa26 LSL)

LSL or conditional expression knockin

Gene replacement KI

Humanized

Full gene replacement

Partial / domain humanized

Domain or partial humanization scope

Immune checkpoint humanized

Checkpoint IO humanization when gene is a checkpoint target

Single humanized

Double / multi humanized

Multi humanized / combination IO project

Transgenic

Random integration transgenic

Safe harbor (ROSA26, HPRT, H11, Col1a1)

Overexpression transgenic

BAC transgenic

BAC transgenic or large fragment insert

Cre / recombinase

Constitutive Cre driver

Inducible Cre (CreER / tet)

Tamoxifen or dox inducible Cre

Dual recombinase (Cre+Dre / Cre+Flp)

Dual recombinase breeding scheme

Flp driver

Flp or FRT derivative allele pairing

Reporter

Reporter knockin (gene specific)

Standalone reporter catalog (lineage tracing, constitutive, dual reporter)

Catalog reporter lines not tied to a single gene allele

Species / background

Rat KO / KI / transgenic

Specify rat on quote

Rabbit models

Specify rabbit on quote

C57BL/6 (B6J / B6N)

Background substrain on quote

BALB/c

129, NOD, NSG, FVB, CD1, DBA/2

Background strain on quote

Mouse Models

Two paths. One scientific team.

Search 14,774+ study ready strains, or commission the exact knockout, knockin, or humanized model your program requires.

Ready to ship

Search 14,774 strains. Many ship from live colonies.

Browse by gene, model type, or therapeutic area. Live colonies available for many top requested knockouts, knockins, and humanized lines.

Trp53Tumor suppressor, knockout & knockin Brca1Breast cancer model, multiple alleles Rosa26Safe harbor for reporters & cassettes

Browse Full Catalog

Model generation

Need a knockout, knockin, or humanized line?

The same U.S. scientific team designs and delivers your allele when it is not on the shelf. 100% germline transmission guarantee.

Conventional and conditional knockouts
Point mutation and humanized knockins
2,800+ models generated since 1998

Request a Quote Explore model generation services

Available Nodal catalog models

1 Nodal line with QC documentation and technical support.

Nodal-KO
Knockout
KO/CKO mice
embryo cryopreservationKO 190536
Order

Selected publications from ingenious targeting laboratory

Peer reviewed work involving Nodal (NODAL) from ITL supported projects.

TBL1XR1 Mutations Drive Extranodal Lymphoma by Inducing a Pro-tumorigenic Memory FateVenturutti L, Teater M, Zhai A, Chadburn A, Babiker L, Kim D, Béguelin W, Lee TC, Kim Y, Chin CR, Yewdell WT, Raught B, Phillip JM, Jiang Y, Staudt LM, Green MR, Chaudhuri J, Elemento O, Farinha P, Weng AP, Nissen MD, Steidl C, Morin RD, Scott DW, Privé GG, Melnick AM. (2020). Cell 182(2): 297-316.e27
Tgif1 and Tgif2 regulate Nodal signaling and are required for gastrulation.Powers SE, Taniguchi K, Yen W, Melhuish TA, Shen J, Walsh CA, Sutherland AE, Wotton D. (2010). Development137: 249-259.1

View all ITL publications

Looking for a Nodal mouse model?

We have 1 Nodal model available — including Knockout types. Contact us today for current availability and our fastest turnaround options. All models come with full QC documentation and technical support.

Best pricing in the industry. Get a quote in 24 hours. Our team of PhD scientists is available to help you select the right model for your research.

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Models Available

Model Types

24 hrs

Quote Turnaround

Included

QC Documentation