Optn Knockout mouse models
Optineurin, encoded by mouse Optn and orthologous to human OPTN, is a coiled-coil autophagy receptor carrying an LC3-interacting region, a UBAN motif that binds linear M1-linked polyubiquitin, and a C-terminal zinc finger. TBK1 phosphorylates optineurin at Ser177 and adjacent residues to enhance LC3 binding and ubiquitin affinity, driving mitophagy downstream of PINK1 and parkin, xenophagy of cytosolic bacteria, and clearance of aggregated protein, while the UBAN domain simultaneously restrains NF-kappaB signaling. Disease logic is allele-specific: E50K acts as a dominant gain of function in normal-tension glaucoma, whereas E478G and truncating alleles cause ALS by abolishing ubiquitin binding. Model designs therefore fork between conditional null, UBAN point knockins such as D477N, phosphomimetic or phosphodead Ser177 variants, and humanization. Because Sqstm1, Nbr1, Tax1bp1, and Calcoco2 provide overlapping receptor activity, expect buffered clearance phenotypes and pair conditional alleles with motor neuron or microglial Cre drivers.
Whole body loss of Optn gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Optn Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Optn knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Optn-KO | Knockout | KO/CKO mice, disease model mice | sperm cryopreservation | KO 225098 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Optn Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Optn allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Optn Knockout mouse models are available?
When Optn Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Optn knockout embryonic lethal in mice?
It depends on background and allele design. Some Optn germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Optn animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Optn?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.