Pck1 Knockin mouse models
Pck1 encodes cytosolic phosphoenolpyruvate carboxykinase, the mouse ortholog of human PCK1, which commits mitochondrially derived oxaloacetate to phosphoenolpyruvate in a GTP-dependent decarboxylation that sets the flux ceiling for hepatic and renal gluconeogenesis and for adipose glyceroneogenesis. Transcription rather than allostery dominates its control: glucagon acting through cAMP and CREB, the glucocorticoid receptor, FOXO1 and PGC-1 alpha drive the promoter, while insulin signaling through AKT suppresses it, making the locus a canonical readout of fasting and insulin resistance. Whole-body deletion is neonatally lethal with massive hepatic steatosis, so albumin-Cre or adipocyte-restricted conditional nulls are required to separate liver from adipose contributions, and promoter-replacement or inducible overexpression alleles test whether transcriptional derepression alone raises glucose output. The mitochondrial paralog Pck2 supplies partial cataplerotic flux but does not rescue cytosolic gluconeogenesis, so compound designs are advisable when quantifying whole-body glucose production.
Targeting Pck1 preserves positional context compared with viral or BAC approaches. That helps when you quantify expression or map chromatin.
What Pck1 Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Pck1 knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Pck1-IRES-CreERT2 | Knockin | Cre/Dre Toolbox of Mice | live | KI 230463 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Pck1 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pck1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pck1 Knockin mouse models are available?
When Pck1 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pck1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Pck1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pck1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pck1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.