Pdcd1 Conditional Knockout mouse — T cell specific context
Pdcd1 encodes PD-1, mouse ortholog of human PDCD1, a single immunoglobulin variable domain inhibitory receptor whose cytoplasmic tail carries an ITIM and, functionally more important, an ITSM that recruits SHP2 to dephosphorylate proximal signaling components, with CD28 rather than the T cell receptor established as the preferred substrate, so PD-1 acts primarily by disabling costimulation. Ligands PD-L1 and PD-L2 are induced by interferon gamma, coupling target cell sensing of inflammation to lymphocyte restraint, and sustained expression marks exhausted CD8 T cells in chronic infection and tumors. Germline nulls develop late, strain dependent autoimmunity, lupus-like nephritis on C57BL/6 and dilated cardiomyopathy on BALB/c, so background must be stated explicitly. Because pembrolizumab and nivolumab do not bind murine PD-1, epitope-matched humanization is mandatory for clinical antibody testing, while conditional nulls with Cd8-Cre address cell intrinsic exhaustion and compound Ctla4 or Lag3 alleles reproduce combination checkpoint synergy.
Conditional deletion of Pdcd1 limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Pdcd1 floxed stock yields usable cohorts. For T cell work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Catalog options
Conditional knockout focuses the experiment on t-cell while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| Pdcd1-Flox | Conditional Knockout | KO/CKO mice | live | CKO 210063 | Inquire |
Designing a Pdcd1 Conditional Knockout allele
The reason to build this rather than use the null is timing and compartment. PD-1 is expressed transiently during thymic selection and on activated B cells, NK cells, and some myeloid populations, so a germline deletion mixes developmental and effector phenotypes. Cd4-Cre already acts at the double positive stage and carries that confound; E8i-Cre restricts loss to CD8 lineage cells after selection, and Ncr1-Cre or Cd19-Cre isolates NK and B contributions. More useful still is an inducible driver such as Gzmb-CreERT2 or a tamoxifen dependent ubiquitous line, which lets you delete after exhaustion is established and ask whether the program is reversible rather than merely preventable. Include tamoxifen-treated Cre-positive controls, since tamoxifen alters lymphocyte numbers. Read out antigen-specific tetramer frequency, TOX, TCF1 and TIM3 co-expression, interferon gamma and TNF production per cell, and tumor or viral load.
Pricing and quotes
The Pdcd1 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pdcd1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with milestones for genotyping, QC, and dispatch.
FAQ
What Pdcd1 Conditional Knockout mouse models are available?
When Pdcd1 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pdcd1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Pdcd1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for t cell specific focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. Your note points to t cell specific, so we start driver selection there.
Do you ship live Pdcd1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pdcd1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.