Pdcd1 Knockin mouse models
Pdcd1 encodes PD-1, mouse ortholog of human PDCD1, a single immunoglobulin variable domain inhibitory receptor whose cytoplasmic tail carries an ITIM and, functionally more important, an ITSM that recruits SHP2 to dephosphorylate proximal signaling components, with CD28 rather than the T cell receptor established as the preferred substrate, so PD-1 acts primarily by disabling costimulation. Ligands PD-L1 and PD-L2 are induced by interferon gamma, coupling target cell sensing of inflammation to lymphocyte restraint, and sustained expression marks exhausted CD8 T cells in chronic infection and tumors. Germline nulls develop late, strain dependent autoimmunity, lupus-like nephritis on C57BL/6 and dilated cardiomyopathy on BALB/c, so background must be stated explicitly. Because pembrolizumab and nivolumab do not bind murine PD-1, epitope-matched humanization is mandatory for clinical antibody testing, while conditional nulls with Cd8-Cre address cell intrinsic exhaustion and compound Ctla4 or Lag3 alleles reproduce combination checkpoint synergy.
An engineered Pdcd1 allele can introduce a human coding region, a point change, or a fluorescent reporter without random transgene integration noise.
What Pdcd1 Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Pdcd1 (PD-1) knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Pdcd1-DTR-2A-tdTomato | Knockin | fluorescent mouse, otherToolbox of Mice(Non-Cre, fluorescent) | embryo cryopreservation | KI 200225 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Pdcd1 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pdcd1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pdcd1 Knockin mouse models are available?
When Pdcd1 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pdcd1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Pdcd1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pdcd1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pdcd1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.