Pdcd1lg2 Conditional Knockout mouse — CD4-Cre pairing
Pdcd1lg2, the mouse ortholog of human PDCD1LG2, encodes PD-L2 (B7-DC), a type I transmembrane protein whose IgV and IgC2 ectodomains engage PD-1 with roughly three-fold higher affinity than PD-L1 while also binding the repulsive guidance molecule RGMB on lung interstitial macrophages. Expression is largely restricted to dendritic cells, macrophages, and germinal center B cells and is driven by IL-4 and STAT6 rather than by the interferon gamma axis that dominates Cd274 induction, so PD-L2 shapes type 2 and tissue-resident tolerance more than interferon-driven adaptive resistance. Allele choice follows directly: conditional nulls isolate the ligand contribution of a defined myeloid or dendritic compartment, whereas ectodomain humanization is required whenever a candidate antibody or PD-1 fusion fails to cross-react with mouse protein. Because Cd274 covers much of the same receptor, single knockouts frequently look silent, so plan Cd274 compound genetics and pair the allele with tumor rejection, exhaustion reversal, or airway inflammation endpoints.
A floxed Pdcd1lg2 allele paired with Cre gives spatial control that whole body knockouts cannot offer. People use it to separate developmental roles from adult homeostasis, to model somatic mutations, and to match disease that begins in one organ. For T cell work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Driver pairing
Labels T cell lineages under Cd4 regulatory elements. Useful when temporal control is less critical than broad T lineage specificity. Compare with inducible CD4 variants if you need late onset deletion.
Catalog options
Conditional knockout focuses the experiment on t-cell while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| Pdcd1lg2-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 220276 | Inquire |
Designing a Pdcd1lg2 Conditional Knockout allele
Because PD-L2 is expressed by a few specific presenting populations rather than broadly, conditional deletion is the only way to attribute a phenotype to a source. Flank the exon encoding the IgV domain so recombination abolishes PD-1 engagement; note that RGMB binding maps to the same ectodomain, so this allele will not separate the two ligand functions. Itgax-Cre covers conventional dendritic cells, Lyz2-Cre the macrophage compartment including the lung interstitial macrophages relevant to the RGMB axis, and Cd19-Cre or Aicda-Cre the germinal center B cell arm, with Aicda-Cre restricting loss to cells that have already been activated. Since IL-4 and STAT6 drive expression, sensitize with house dust mite or ovalbumin before scoring, or the protein will be near absent at baseline. Endpoints are airway hyperresponsiveness and eosinophilia, follicular helper and germinal center output, and antigen-specific CD8 expansion.
Pricing and quotes
The Pdcd1lg2 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pdcd1lg2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with milestones for genotyping, QC, and dispatch.
FAQ
What Pdcd1lg2 Conditional Knockout mouse models are available?
When Pdcd1lg2 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pdcd1lg2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Pdcd1lg2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for cd4 cre focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. Your note points to cd4 cre, so we start driver selection there.
Do you ship live Pdcd1lg2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pdcd1lg2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.