Pdcd1lg2 Knockout mouse models
Pdcd1lg2, the mouse ortholog of human PDCD1LG2, encodes PD-L2 (B7-DC), a type I transmembrane protein whose IgV and IgC2 ectodomains engage PD-1 with roughly three-fold higher affinity than PD-L1 while also binding the repulsive guidance molecule RGMB on lung interstitial macrophages. Expression is largely restricted to dendritic cells, macrophages, and germinal center B cells and is driven by IL-4 and STAT6 rather than by the interferon gamma axis that dominates Cd274 induction, so PD-L2 shapes type 2 and tissue-resident tolerance more than interferon-driven adaptive resistance. Allele choice follows directly: conditional nulls isolate the ligand contribution of a defined myeloid or dendritic compartment, whereas ectodomain humanization is required whenever a candidate antibody or PD-1 fusion fails to cross-react with mouse protein. Because Cd274 covers much of the same receptor, single knockouts frequently look silent, so plan Cd274 compound genetics and pair the allele with tumor rejection, exhaustion reversal, or airway inflammation endpoints.
Whole body loss of Pdcd1lg2 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Pdcd1lg2 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Pdcd1lg2 (PD-L2) knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Designing a Pdcd1lg2 Knockout allele
The single null is viable and usually silent, which is the main design fact to plan around. Delete the exons encoding the IgV and IgC2 domains so no soluble ectodomain remains to act as a decoy for PD-1. The important practical point is linkage: Cd274 sits immediately adjacent to Pdcd1lg2 on mouse chromosome 19, so a double-deficient animal cannot be produced by simply intercrossing two independently targeted lines, because the required cis configuration depends on a rare recombination between tightly linked loci. Target both genes in the same zygote instead, or screen intensively for the recombinant chromosome and confirm it by long-range PCR. Baseline immunophenotyping will be unremarkable, so plan challenge: allergic airway inflammation, chronic viral infection, and syngeneic tumor rejection, using response to PD-1 blockade as a functional measure of remaining ligand supply.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Pdcd1lg2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pdcd1lg2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pdcd1lg2 Knockout mouse models are available?
When Pdcd1lg2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pdcd1lg2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Pdcd1lg2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pdcd1lg2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pdcd1lg2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.