Pdha1 Knockout mouse models
Pdha1, the mouse ortholog of human PDHA1, encodes the E1 alpha subunit of the pyruvate dehydrogenase complex, which pairs with E1 beta in an alpha2beta2 heterotetramer, binds thiamine pyrophosphate, and performs the decarboxylation of pyruvate that commits carbon to acetyl-CoA and the TCA cycle. Regulation runs through three serine residues on this subunit that are phosphorylated by the PDK kinases and reversed by PDP phosphatases, making the protein the direct node where nutrient state gates glycolytic carbon entry into oxidative metabolism. The gene is X-linked, and PDHA1 deficiency is the commonest cause of congenital lactic acidosis, hemizygous in males and mosaic in heterozygous females, an architecture that should shape design: conditional floxed alleles permit tissue-restricted loss without the confound of random X inactivation, while patient-matched knockins retain residual activity for dichloroacetate or ketogenic diet studies. No paralog compensates. Select neuronal or hepatic Cre drivers according to neuropathology or systemic lactate endpoints.
Pdha1 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Pdha1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Pdha1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Pdha1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 250966 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Pdha1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pdha1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pdha1 Knockout mouse models are available?
When Pdha1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pdha1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Pdha1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pdha1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pdha1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.