READY TO SHIP!
DEVELOPING
Pfkm Conditional Knockout mouse models
Skeletal muscle glycolytic capacity rests on Pfkm, the mouse ortholog of human PFKM, which encodes the muscle subunit of phosphofructokinase 1 and dominates the tetramer in that tissue, where it catalyzes the committed, effectively irreversible ATP-dependent phosphorylation of fructose 6-phosphate and integrates AMP, ATP, citrate and fructose 2,6-bisphosphate signals into glycolytic rate. Recessive loss causes Tarui disease, glycogen storage disease type VII, in which exercise intolerance, cramps, myoglobinuria and compensated hemolysis follow from blocked muscle glycolysis, with the erythrocyte phenotype partial rather than complete because red cells contain M and L hybrid tetramers. Model design follows that tissue logic: a germline or muscle-restricted conditional null reproduces the metabolic myopathy, while patient-matched missense or splice knockins model residual activity and milder late-onset forms. Redundancy with Pfkl and Pfkp is real outside muscle. Choose the allele by endpoint, whether exercise challenge, glycogen accumulation, phosphorus magnetic resonance spectroscopy or hemolytic indices are the primary measures.
Pfkm conditional knockout mice carry a floxed allele so you delete function only where Cre is active. The germline allele stays intact until you cross to a tissue specific Cre. Labs reach for this design when a global knockout is lethal, when they need adult onset loss, or when regional redundancy hides a whole body phenotype.
What Pfkm Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Pfkm conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Pfkm-Flox | CKO | CKO | DEVELOPING | CKO 262882 | Inquire |
| These mice carry loxP sites flanking exon3-4 of Pfkm gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Pfkm gene. | |||||
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Pfkm Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pfkm allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pfkm Conditional Knockout mouse models are available?
When Pfkm Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pfkm knockout embryonic lethal in mice?
It depends on background and allele design. Some Pfkm germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pfkm animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pfkm?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Related models and routes
Breed this line with ingenious targeting laboratory
Send the Pfkm Conditional Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.