Pik3ca Knockin mouse models
Pik3ca, the mouse ortholog of PIK3CA, encodes p110 alpha, the class IA PI3K catalytic subunit that heterodimerizes with p85 regulatory subunits and phosphorylates PIP2 to PIP3 downstream of receptor tyrosine kinases and RAS, nucleating PDPK1 and AKT recruitment at the membrane. Oncogenic substitutions cluster in two structural classes with distinct mechanisms: helical domain changes E542K and E545K relieve the p85 nSH2 inhibitory contact, whereas the kinase domain change H1047R alters the membrane-binding interface, and the same alleles cause PIK3CA-related overgrowth spectrum when mosaic. Cre-activated latent knockins such as a lox-stop-lox H1047R allele are therefore standard for modeling dose and timing of activation, kinase-dead knockins isolate lipid kinase output from scaffolding, and conditional nulls test tissue dependence, since germline deletion is embryonic lethal at midgestation. Because p110 beta sustains PIP3 in some lineages, interpret isoform-selective inhibitor studies against compound Pik3ca and Pik3cb genetics.
Pik3ca knockin models place a defined sequence at the endogenous locus. Expression stays under native regulation, which matters for reporters, tags, and precise allele swaps.
What Pik3ca Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Pik3ca knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Pik3ca-LSL-E545K | Knockin | point mutantion mice, disease model mice | live | KI 232348 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Pik3ca Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pik3ca allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pik3ca Knockin mouse models are available?
When Pik3ca Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pik3ca knockout embryonic lethal in mice?
It depends on background and allele design. Some Pik3ca germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pik3ca animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pik3ca?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.