Pkm Knockout mouse models
Pkm, the mouse ortholog of human PKM, encodes pyruvate kinase M and is regulated principally by mutually exclusive splicing of exon 9 versus exon 10, controlled by PTBP1 and by hnRNPA1 and hnRNPA2, yielding either the constitutively active PKM1 tetramer typical of differentiated tissue or PKM2, whose tetramer to dimer equilibrium responds to fructose 1,6-bisphosphate, serine, phosphotyrosine peptides and acetylation. The low-activity PKM2 dimer throttles the terminal glycolytic step, backing upstream intermediates into biosynthetic branches and underpinning the Warburg phenotype, with additional nuclear functions reported. Consequently, isoform-selective engineering matters more than deletion: exon-specific alleles that force PKM1 or PKM2 expression test the splicing hypothesis directly, while conditional nulls address whether the locus is required at all in a given lineage. The Pklr paralog covers liver and erythroid tissue only. Match the allele to lactate flux, biosynthetic tracing or tumor initiation endpoints with tissue-matched Cre drivers.
A conventional Pkm knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Pkm Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Pkm knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Pkm-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 205060 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Pkm Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pkm allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pkm Knockout mouse models are available?
When Pkm Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pkm knockout embryonic lethal in mice?
It depends on background and allele design. Some Pkm germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pkm animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pkm?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.