Pla2g6 Knockout mouse models
Membrane phospholipid remodeling depends on Pla2g6, the mouse ortholog of human PLA2G6, encoding calcium-independent phospholipase A2 beta, an ankyrin repeat containing enzyme with a canonical GXSXG lipase motif that hydrolyzes the sn-2 acyl chain of glycerophospholipids to release fatty acids and lysophospholipids. This activity governs membrane fatty acid remodeling, mitochondrial and ER membrane integrity, store-operated calcium signaling, and arachidonate supply. Recessive mutations cause infantile neuroaxonal dystrophy, atypical neuroaxonal dystrophy, and PARK14 dystonia-parkinsonism, with axonal spheroids, tubulovesicular membrane accumulation, and variable brain iron deposition. Usefully, Pla2g6 knockout and patient-type missense knockin mice do develop progressive spheroid pathology and motor decline, making constitutive null a legitimate starting allele, while catalytically dead knockins separate loss of hydrolase activity from dominant-negative effects. Conditional alleles crossed to neuronal or dopaminergic Cre lines are preferable when regional axonal degeneration rather than systemic lipid change is the endpoint.
Pla2g6 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Pla2g6 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Pla2g6 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Pla2g6-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 200413 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Pla2g6 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pla2g6 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pla2g6 Knockout mouse models are available?
When Pla2g6 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pla2g6 knockout embryonic lethal in mice?
It depends on background and allele design. Some Pla2g6 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pla2g6 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pla2g6?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.