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Plau Conditional Knockout mouse models
Encoding urokinase-type plasminogen activator, Plau is the mouse ortholog of human PLAU and produces a single-chain zymogen that binds its GPI-anchored receptor uPAR (Plaur) through an N-terminal growth factor domain, is activated by plasmin or cathepsin cleavage, and then generates plasmin at the cell surface rather than in the fluid phase. This pericellular proteolysis drives matrix degradation, latent TGF-beta and pro-MMP activation, macrophage and smooth muscle migration, and integrin plus vitronectin dependent signaling that persists even when catalysis is blocked. Consequently a conditional null tests requirement in myeloid, endothelial, or tumor compartments, an active-site knockin separates proteolysis from uPAR occupancy, and humanization of the growth factor domain is necessary for antibodies or ATF fusions that ignore mouse protein. Plat and plasma kallikrein compensate in the fluid phase, so pair Plau alleles with invasion, fibrosis, or fibrinolysis endpoints and a validated lineage Cre.
Plau conditional knockout mice carry a floxed allele so you delete function only where Cre is active. The germline allele stays intact until you cross to a tissue specific Cre. Labs reach for this design when a global knockout is lethal, when they need adult onset loss, or when regional redundancy hides a whole body phenotype.
What Plau Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Plau conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Plau-Flox | CKO | CKO | IN PRODUCTION | CKO 253996 | Inquire |
| These mice carry loxP sites flanking exon 3-4 of Plau gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Plau gene. | |||||
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Plau Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Plau allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Plau Conditional Knockout mouse models are available?
When Plau Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Plau knockout embryonic lethal in mice?
It depends on background and allele design. Some Plau germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Plau animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Plau?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ingenious targeting laboratory
Send the Plau Conditional Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.