READY TO SHIP!
IN PRODUCTION
Pml Conditional Knockout mouse models
Pml encodes the mouse ortholog of human PML (TRIM19), a RBCC tripartite motif protein whose coiled coil and SUMO to SIM interactions polymerize into PML nuclear bodies that concentrate p53, CBP, HIPK2, DAXX, and SP100 to coordinate apoptosis, senescence, antiviral responses, and stem cell quiescence. Its cardinal oncogenic role is as the 5 prime partner in t(15;17) PML-RARA, the driver of acute promyelocytic leukemia, where the fusion oligomerizes through the PML coiled coil, converts retinoic acid receptor alpha into a constitutive repressor of myeloid differentiation genes, and is uniquely susceptible to all-trans retinoic acid and to arsenic trioxide, which binds PML cysteines and triggers SUMO-dependent RNF4 degradation. Germline nulls are viable but tumor prone with impaired senescence, so conditional nulls address tumor suppressor and stem cell questions, whereas cathepsin G promoter driven or inducible PML-RARA knockins generate authentic promyelocytic leukemia. Because arsenic targeting depends on precise cysteine and SUMO site context, humanized PML alleles suit pharmacology endpoints.
Tissue restricted knockout of Pml keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells.
What Pml Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Pml conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Pml-Flox | CKO | CKO | IN PRODUCTION | CKO 260145 | Inquire |
| These mice carry loxP sites flanking exon 2 of Pml gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Pml gene. | |||||
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Pml Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pml allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pml Conditional Knockout mouse models are available?
When Pml Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pml knockout embryonic lethal in mice?
It depends on background and allele design. Some Pml germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pml animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pml?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Related models and routes
Breed this line with ingenious targeting laboratory
Send the Pml Conditional Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.