Pparg Conditional Knockout mouse — adipocyte specific context
Adipocyte identity depends on Pparg, the mouse ortholog of PPARG, a ligand-activated nuclear receptor that heterodimerizes with RXR alpha and occupies PPRE elements through tandem zinc fingers, while its ligand-binding domain accommodates fatty acid derivatives and thiazolidinediones and exchanges NCoR corepressor for coactivators upon agonist binding. Insulin sensitization additionally depends on blocking CDK5 and ERK phosphorylation of Ser273, which reprograms rather than simply activates the receptor. The PPARG2 isoform, carrying an extra amino-terminal segment from an upstream promoter, is adipose-restricted, so isoform-specific alleles ask different questions than pan-locus deletion. Germline null is embryonic lethal from placental vascular failure, making adipocyte, macrophage or intestinal conditional nulls mandatory, while dominant-negative knockins such as P467L reproduce familial partial lipodystrophy and Ser273 phospho-dead alleles isolate the antidiabetic mechanism. Ppara and Ppard bind overlapping ligands but cannot restore adipogenesis, so compensation is confined to lipid oxidation endpoints.
Conditional deletion of Pparg limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Pparg floxed stock yields usable cohorts. For adipocyte work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Catalog options
Conditional knockout focuses the experiment on adipocyte while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| Pparg-Flox | Conditional Knockout | KO/CKO mice, disease model mice | live | CKO 190071 | Inquire |
Designing a Pparg Conditional Knockout allele
Germline deletion is off the table because of placental failure, so every question about this receptor in adults runs through a floxed allele. Flank the exons encoding the tandem zinc fingers so recombination removes DNA binding rather than only the ligand pocket, which prevents a receptor that still occupies PPRE sites without ligand response. Match the driver to the compartment: Adipoq-Cre for mature adipocytes, Pdgfra-CreERT2 for preadipocyte recruitment, Ucp1-CreERT2 for brown and beige fat, LysM-Cre or Itgax-Cre for macrophage polarization, Cd4-Cre for visceral regulatory T cells, and Villin-Cre for intestinal epithelium. Avoid Fabp4-Cre, which recombines embryonically and in macrophages and has generated conflicting adipose literature. Deleting in mature adipocytes triggers cell death and progressive lipodystrophy, so separate acute signaling loss from tissue loss by timed tamoxifen. Read fat pad mass, adipocyte diameter, serum adiponectin, ectopic lipid, glucose tolerance, and loss of thiazolidinedione response.
Pricing and quotes
The Pparg Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pparg allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with milestones for genotyping, QC, and dispatch.
FAQ
What Pparg Conditional Knockout mouse models are available?
When Pparg Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pparg knockout embryonic lethal in mice?
It depends on background and allele design. Some Pparg germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for adipocyte specific focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. Your note points to adipocyte specific, so we start driver selection there.
Do you ship live Pparg animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pparg?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.