Pparg Xenograft-Applicable mouse models
Adipocyte identity depends on Pparg, the mouse ortholog of PPARG, a ligand-activated nuclear receptor that heterodimerizes with RXR alpha and occupies PPRE elements through tandem zinc fingers, while its ligand-binding domain accommodates fatty acid derivatives and thiazolidinediones and exchanges NCoR corepressor for coactivators upon agonist binding. Insulin sensitization additionally depends on blocking CDK5 and ERK phosphorylation of Ser273, which reprograms rather than simply activates the receptor. The PPARG2 isoform, carrying an extra amino-terminal segment from an upstream promoter, is adipose-restricted, so isoform-specific alleles ask different questions than pan-locus deletion. Germline null is embryonic lethal from placental vascular failure, making adipocyte, macrophage or intestinal conditional nulls mandatory, while dominant-negative knockins such as P467L reproduce familial partial lipodystrophy and Ser273 phospho-dead alleles isolate the antidiabetic mechanism. Ppara and Ppard bind overlapping ligands but cannot restore adipogenesis, so compensation is confined to lipid oxidation endpoints.
Pparg engineered hosts can be tuned for xenograft take rate and growth kinetics depending on tumor lineage.
What Pparg Xenograft-Applicable mouse models are available?
ingenious targeting laboratory offers 1 distinct Pparg xenograft-applicable catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Pparg-Flox/Cx3cr1-CreERT2 | Xenograft-Applicable | KO/CKO mice,Cre/Dre Toolbox of Mice | live | XA 241654 | Inquire |
Why this approach
We help teams pick alleles that match the experimental question, the organ system, and future breeding plans. For your target tissue centric work, Cre specificity, flox placement, and baseline controls matter as much as the gene edit itself.
Pricing and quotes
The Pparg Xenograft-Applicable lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pparg allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pparg Xenograft-Applicable mouse models are available?
When Pparg Xenograft-Applicable lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pparg knockout embryonic lethal in mice?
It depends on background and allele design. Some Pparg germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pparg animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pparg?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.