Proc Knockout mouse models
Encoding protein C, Proc is the mouse ortholog of human PROC and yields a vitamin K dependent zymogen with a Gla domain, two EGF modules, and a chymotrypsin-like protease domain that is activated when thrombin, presented by endothelial thrombomodulin and concentrated by EPCR, removes the activation peptide. Activated protein C then uses protein S as cofactor to proteolyze factors Va and VIIIa, shutting down thrombin amplification, and separately signals through EPCR-dependent PAR1 cleavage to stabilize endothelial barrier function and dampen NF-kB driven cytokine output. Complete deficiency in mice is perinatal lethal from consumptive coagulopathy, so severe models rely on hypomorphic alleles giving a few percent of normal activity, hepatocyte-restricted conditional deletion with Alb-Cre, or signaling-selective knockins that retain cytoprotection without anticoagulant function. Select by endpoint, whether microvascular thrombosis, sepsis survival, or permeability, since the anticoagulant and cytoprotective arms segregate genetically.
A conventional Proc knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Proc Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Proc knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Proc-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 202108 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Proc Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Proc allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Proc Knockout mouse models are available?
When Proc Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Proc knockout embryonic lethal in mice?
It depends on background and allele design. Some Proc germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Proc animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Proc?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.