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Psen2 Conditional Knockout mouse models
Second catalytic subunit of gamma-secretase, Psen2 is the mouse ortholog of human PSEN2 and assembles with nicastrin, APH1, and PEN2 into complexes localizing preferentially to late endosomes and lysosomes rather than the plasma membrane, giving PSEN2-containing gamma-secretase a distinct intracellular substrate pool and a documented role in mitochondria-associated ER membrane calcium handling. Rare dominant PSEN2 mutations, including N141I, cause early onset Alzheimer disease by shifting cleavage toward longer amyloid beta species. Unlike Psen1, Psen2 knockout mice are viable and fertile with only mild age-dependent pulmonary fibrosis, which makes constitutive null tractable, whereas Psen1 and Psen2 double nulls phenocopy severe Notch deficiency and die embryonically. Genuine redundancy with Psen1 therefore dictates a compound conditional strategy whenever total gamma-secretase output is the endpoint. Use patient knockins for amyloid ratio questions, conditional double alleles for substrate accumulation studies, and humanization when modulator pharmacology is the goal.
Conditional deletion of Psen2 limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Psen2 floxed stock yields usable cohorts.
What Psen2 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Psen2 conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Psen2-Flox | CKO | CKO, Disease Model | IN PRODUCTION | CKO 253856 | Inquire |
| These mice carry loxP sites flanking exon 3 of Psen2 gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Psen2 gene. | |||||
Designing a Psen2 Conditional Knockout allele
A conditional at this locus is nearly silent alone, because Psen1 carries most cleavage in almost every tissue examined. Design it as half of a compound strategy, floxing the exons encoding the catalytic core and combining with a Psen1 conditional; the two genes sit on different mouse chromosomes, so budget the breeding and cohort mathematics accordingly. Where the allele earns its place by itself is compartment-specific biology, since these complexes concentrate in late endosomes and lysosomes. Cx3cr1-CreERT2 for microglial phagosome maturation or Sftpc-CreERT2 for alveolar epithelium addresses questions Psen1 deletion cannot. Tamoxifen timing matters in lung, where the fibrotic phenotype is age dependent. Read endolysosomal pH and cathepsin maturation, calcium transfer at endoplasmic reticulum to mitochondria contacts, and C99 and Notch intracellular domain levels, always quantifying Psen1 transcript for compensation.
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Psen2 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Psen2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Psen2 Conditional Knockout mouse models are available?
When Psen2 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Psen2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Psen2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Psen2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Psen2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ingenious targeting laboratory
Send the Psen2 Conditional Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.