Rb1 Xenograft-Applicable mouse models
The prototypic tumor suppressor Rb1, ortholog of human RB1, encodes a pocket protein whose A and B domains form a cleft that binds the transactivation domain of E2F1 through E2F3 and, through a separate LXCXE-binding groove, recruits histone deacetylases and chromatin modifiers as well as viral oncoproteins including E7 and large T antigen. Sequential phosphorylation by cyclin D with CDK4 or CDK6 and then cyclin E with CDK2 releases E2F and licenses S phase entry, the axis targeted by palbociclib, and biallelic loss causes retinoblastoma and is pervasive in small cell lung cancer. Germline deletion is embryonic lethal from placental and erythroid defects, so conditional nulls are standard, and phosphorylation site or LXCXE-cleft knockins separate CDK regulation from chromatin recruitment. Critically, the paralogs Rbl1 and Rbl2 compensate robustly in mouse retina and elsewhere, so compound conditional genetics is usually required to reproduce the human tumor spectrum.
Orthotopic and subcutaneous routes both benefit from consistent Rb1 genetics across cages.
What Rb1 Xenograft-Applicable mouse models are available?
ingenious targeting laboratory offers 1 distinct Rb1 xenograft-applicable catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Rb1-Flox/Trp53-Flox/Pten-Flox | Xenograft-Applicable | KO/CKO mice | embryo cryopreservation | XA 240019 | Inquire |
Why this approach
We help teams pick alleles that match the experimental question, the organ system, and future breeding plans. For your target tissue centric work, Cre specificity, flox placement, and baseline controls matter as much as the gene edit itself.
Pricing and quotes
The Rb1 Xenograft-Applicable lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Rb1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Rb1 Xenograft-Applicable mouse models are available?
When Rb1 Xenograft-Applicable lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Rb1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Rb1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Rb1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Rb1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.