Rps6kb1 Conditional Knockout mouse models
S6 kinase 1, encoded by Rps6kb1 and orthologous to human RPS6KB1, is an AGC kinase recruited to mTORC1 through a TOS motif that binds RAPTOR, then activated by mTOR phosphorylation of the Thr389 hydrophobic motif followed by PDPK1 phosphorylation of Thr229 in the activation loop. Its substrates include ribosomal protein S6, eIF4B, PDCD4, eEF2 kinase and IRS-1, the last constituting the negative feedback loop that limits PI3K signaling and underlies paradoxical insulin sensitization by rapamycin. Nulls are viable but small with hypoinsulinemia and reduced beta cell size, so conditional alleles matter mainly for tissue-autonomous growth questions, while a Thr389 phospho-dead knockin isolates mTORC1 input from PDPK1 input in a way inhibitors cannot. Because Rps6kb2 phosphorylates overlapping substrates and is upregulated after Rps6kb1 loss, compound alleles or an Rps6 phospho-dead knockin should be planned when translation output is the endpoint.
Tissue restricted knockout of Rps6kb1 keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells.
What Rps6kb1 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Rps6kb1 conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Rps6kb1-Flox | Conditional Knockout | KO/CKO mice | live | CKO 241277 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Rps6kb1 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Rps6kb1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Rps6kb1 Conditional Knockout mouse models are available?
When Rps6kb1 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Rps6kb1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Rps6kb1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Rps6kb1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Rps6kb1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.