Rps6kb2 Knockout mouse models
Rps6kb2 encodes S6 kinase 2, the ortholog of human RPS6KB2, an AGC kinase that shares the TOS motif, RAPTOR docking and mTORC1-dependent hydrophobic motif phosphorylation with S6K1 but carries a carboxy-terminal nuclear localization signal, lacks the amino-terminal extension that targets S6K1 to the nucleolus, and accounts for a substantial fraction of ribosomal protein S6 phosphorylation in many tissues. Single deletion produces essentially no growth phenotype and is accompanied by increased S6K1 activity, a compensatory relationship that is the central design consideration here, since combined Rps6kb1 and Rps6kb2 deletion causes perinatal lethality and abolishes S6 phosphorylation. Practical strategies therefore use conditional alleles at both loci with a common Cre driver, or an Rps6 serine to alanine knockin when the question concerns S6 phosphorylation rather than kinase identity. Reserve single-locus nulls for pharmacological specificity controls when validating pan-S6K inhibitors in a defined tissue.
A conventional Rps6kb2 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Rps6kb2 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Rps6kb2 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Rps6kb2-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 191118 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Rps6kb2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Rps6kb2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Rps6kb2 Knockout mouse models are available?
When Rps6kb2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Rps6kb2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Rps6kb2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Rps6kb2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Rps6kb2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.