Rptor Knockout mouse models
Raptor, encoded by Rptor and orthologous to human RPTOR, is the substrate-presenting scaffold of mTOR complex 1, recognizing TOS motifs in S6K1, 4E-BP1 and other clients, tethering the complex to the lysosomal surface through the Rag GTPase heterodimer and Ragulator during amino acid sufficiency, and providing the surface required for FKBP12-rapamycin inhibition. It is also the node where catabolic input converges, since AMPK phosphorylation at Ser792 creates 14-3-3 binding sites that suppress mTORC1 independently of RHEB. Germline deletion is early embryonic lethal, so conditional nulls with muscle, cardiac, hepatic or immune Cre drivers are the standard tool for testing complex-specific requirements, and matched Rictor conditional cohorts distinguish mTORC1 from mTORC2 contributions. A Ser792 phospho-dead knockin isolates AMPK control from nutrient control, which is the appropriate allele when the endpoint is metabolic stress rather than growth factor signaling.
Global Rptor deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Rptor Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Rptor knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Rptor-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 210185 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Rptor Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Rptor allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Rptor Knockout mouse models are available?
When Rptor Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Rptor knockout embryonic lethal in mice?
It depends on background and allele design. Some Rptor germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Rptor animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Rptor?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.