Runx1 Knockout mouse models
Definitive hematopoiesis requires Runx1, the mouse ortholog of human RUNX1 (AML1), a Runt domain transcription factor that heterodimerizes with CBFB to raise DNA affinity at core enhancer TGTGGT motifs and recruits either coactivators such as CBP or corepressors including NCOR-HDAC and GFI1 machinery, governing endothelial to hematopoietic transition, megakaryocyte maturation, and lymphoid development. Disease alleles are mechanistically diverse: germline heterozygous mutations cause familial platelet disorder with predisposition to myeloid malignancy, somatic point mutations cluster in the Runt domain in myelodysplastic syndrome and acute myeloid leukemia, and translocations generate RUNX1-RUNX1T1 or ETV6-RUNX1 chimeras that convert the factor into a dominant repressor. Because germline nulls die around midgestation lacking definitive hematopoietic stem cells, conditional nulls with Vav1-cre or Mx1-cre are essential, while knockin of Runt domain point mutants or endogenous fusion alleles addresses dominant negative mechanism. Paralogs Runx2 and Runx3 share CBFB and DNA motifs, so lineage compensation should be tested explicitly.
Runx1 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Runx1 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Runx1 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Runx1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Runx1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Runx1 Knockout mouse models are available?
When Runx1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Runx1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Runx1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Runx1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Runx1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.