S100a9 Knockout mouse models
Encoding MRP14 (calgranulin B), S100a9 is the mouse ortholog of human S100A9 and pairs with S100A8 through a hydrophobic interface to build the calprotectin heterodimer, then assembles into calcium-dependent tetramers that are the receptor-active species for TLR4 and RAGE. The protein carries a C-terminal extension with an additional metal site and a phosphorylation-regulated segment, and it directs the complex to membranes and to the NADPH oxidase during neutrophil activation, while extracellular tetramers drive chemotaxis, beta 2 integrin affinity maturation, and metal sequestration that restricts microbial growth. Nulls are viable but lose S100A8 protein as well, which confounds subunit-specific conclusions and argues for tetramerization-deficient or metal-site knockins alongside myeloid conditional alleles. Because mice lack S100a12, calgranulin biology cannot be fully reconstructed without human transgenes. Match the allele to endpoints such as sepsis mortality, autoimmune arthritis, or tumor myeloid suppression.
A conventional S100a9 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What S100a9 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct S100a9 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| S100a9-KO | Knockout | KO/CKO mice | live | KO 190143 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The S100a9 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a S100a9 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What S100a9 Knockout mouse models are available?
When S100a9 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is S100a9 knockout embryonic lethal in mice?
It depends on background and allele design. Some S100a9 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live S100a9 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for S100a9?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.