Scn1a Knockin mouse models
Interneuron excitability in mouse depends on Scn1a, ortholog of human SCN1A, encoding the Nav1.1 pore-forming alpha subunit assembled from four homologous domains, each contributing six transmembrane segments with an S4 voltage sensor and a re-entrant P loop, plus the DIII to DIV linker carrying the IFM fast inactivation particle. Nav1.1 is enriched in the axon initial segment of parvalbumin and somatostatin interneurons, so haploinsufficiency preferentially silences inhibition and produces the disinhibition seen in Dravet syndrome, while milder missense alleles cause GEFS+ and specific gain of function variants cause familial hemiplegic migraine 3. Allele class maps precisely onto question: heterozygous conditional null models Dravet dosage, patient knockins such as R1648H or A1783V test channel biophysics, and humanized alleles enable antisense or gene therapy testing. Homozygous nulls die by roughly postnatal day 15 and seizure severity is strongly background dependent, so fix strain and choose interneuron-restricted Cre carefully.
Knockin designs at Scn1a support head to head comparisons between wild type and modified alleles because regulatory DNA stays in place.
What Scn1a Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Scn1a knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Scn1a-c.473+5G>A | Knockin | point mutantion mice | sperm cryopreservation | KI 210417 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Scn1a Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Scn1a allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Scn1a Knockin mouse models are available?
When Scn1a Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Scn1a knockout embryonic lethal in mice?
It depends on background and allele design. Some Scn1a germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Scn1a animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Scn1a?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.