Serpinf1 Knockout mouse models
Serpinf1, the mouse ortholog of human SERPINF1, encodes pigment epithelium derived factor, a non-inhibitory serpin whose reactive center loop is proteolytically nonfunctional and which instead uses an asymmetric charge distribution to bind collagen I and glycosaminoglycans on one face and laminin receptor plus PNPLA2 on the other. Through those interactions PEDF acts as a potent endogenous antiangiogenic and neurotrophic factor, inducing endothelial FAS dependent apoptosis and dampening VEGF signaling, while in bone it is required for osteoid mineralization, so recessive human loss of function causes osteogenesis imperfecta type VI with normal collagen but defective matrix mineralization. Nulls are viable and reproduce low bone mass with increased retinal and pancreatic vascularity, making germline deletion useful, while receptor-binding-domain knockins separate antiangiogenic from lipase-modulating and neurotrophic activities and conditional alleles isolate retinal pigment epithelium or osteoblast sources. Anchor the allele to choroidal neovascularization, bone mineral density, or photoreceptor survival endpoints.
Serpinf1 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Serpinf1 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Serpinf1 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Serpinf1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Serpinf1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Serpinf1 Knockout mouse models are available?
When Serpinf1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Serpinf1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Serpinf1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Serpinf1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Serpinf1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.