Sf3b1 Knockout mouse models
Splicing fidelity depends on Sf3b1, the mouse ortholog of human SF3B1, the largest subunit of the SF3b module of the U2 small nuclear ribonucleoprotein, built from twenty HEAT repeats that clamp the pre-messenger RNA branch point adenosine and stabilize U2 snRNA duplex formation during A complex assembly, with PHF5A and SF3B3 completing the pocket targeted by pladienolide and related modulators. Cancer-associated mutations are heterozygous, mutually exclusive missense changes, chiefly K700E in myelodysplastic syndrome with ring sideroblasts and in chronic lymphocytic leukemia, and R625 substitutions in uveal melanoma, acting as change of function by promoting cryptic 3 prime splice site selection and nonsense-mediated decay of transcripts such as Abcb7 and Map3k7. Consequently, conditional heterozygous hotspot knockins are the only faithful design, since homozygous mutant and null alleles are lethal, and induction with Mx1-cre or Vav1-cre reproduces dysplastic erythropoiesis. Match allele class to endpoint, whether mis-splicing catalogs, sideroblastic phenotype, or spliceosome modulator sensitivity.
Global Sf3b1 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Sf3b1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Sf3b1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Sf3b1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 250116 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Sf3b1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Sf3b1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Sf3b1 Knockout mouse models are available?
When Sf3b1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Sf3b1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Sf3b1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Sf3b1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Sf3b1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.