Shank3 Knockout mouse models
Deletion of Shank3, the mouse ortholog of human SHANK3, is the principal genetic driver of Phelan-McDermid syndrome arising from 22q13.3 loss, and the gene encodes the most abundant SHANK scaffold in striatal and cortical postsynaptic densities, with ankyrin repeats, SH3, a PDZ domain binding GKAP/SAPAP, proline-rich Homer and cortactin sites, and a SAM domain that polymerizes the PSD lattice. Multiple intragenic promoters and extensive alternative splicing generate isoforms with distinct localization, which is why different exon-specific mouse lines produce divergent synaptic and behavioral phenotypes. Haploinsufficiency reduces AMPA and NMDA receptor content, impairs corticostriatal transmission, and produces repetitive grooming and social deficits. Allele planning must specify exact exons, choosing conditional null for reversibility studies, isoform-selective deletion matched to patient breakpoints, truncating knockins, or humanization for oligonucleotide and gene therapy testing. Partial redundancy with Shank1 and Shank2 argues for compound alleles when total scaffold capacity is the endpoint.
A conventional Shank3 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Shank3 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Shank3 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Shank3-KO | Knockout | KO/CKO mice, disease model mice | sperm cryopreservation | KO 241043 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Shank3 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Shank3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Shank3 Knockout mouse models are available?
When Shank3 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Shank3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Shank3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Shank3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Shank3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.