Shc1 Knockout mouse models
Three isoforms, p46, p52 and p66, arise from Shc1, the ortholog of human SHC1, through alternative translation initiation and promoter use; all share a phosphotyrosine-binding domain and an SH2 domain that engage NPXY motifs on activated receptor tyrosine kinases, plus a CH1 region whose Tyr239, Tyr240 and Tyr317 phosphorylation recruits GRB2 and SOS1 to load RAS and activate ERK. The p66 isoform is functionally distinct, carrying a CH2 extension whose Ser36 phosphorylation under oxidative stress promotes mitochondrial translocation, cytochrome c oxidation and hydrogen peroxide generation, linking the locus to oxidative stress resistance and lifespan. Because pan-locus deletion is embryonic lethal with cardiovascular defects, adult questions require conditional nulls, whereas isoform-selective p66 knockouts are viable and are the correct allele for aging and redox endpoints. The paralogs Shc2, Shc3 and Shc4 have restricted neural and muscle expression and do not broadly compensate, so tissue context should guide interpretation.
Whole body loss of Shc1 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Shc1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Shc1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Shc1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 231739 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Shc1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Shc1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Shc1 Knockout mouse models are available?
When Shc1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Shc1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Shc1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Shc1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Shc1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.