Shh Knockin mouse models
Shh is the mouse ortholog of human SHH and encodes Sonic hedgehog, which autoprocesses to attach cholesterol to the carboxy terminus of its signaling fragment and receives palmitate at Cys24 from HHAT, then depends on DISP1 and SCUBE2 for release before binding PTCH1 with the coreceptors CDON, BOC, and GAS1 to relieve SMO inhibition and shift ciliary GLI processing from GLI3 repressor toward activator forms. It patterns the ventral neural tube as floor plate signal and specifies digit identity from the zone of polarizing activity, and human mutations cause holoprosencephaly type 3 while pathway-activating PTCH1 and SMO lesions drive basal cell carcinoma and medulloblastoma. Nulls die with cyclopia and severe midline defects, so conditional deletion, holoprosencephaly missense knockins, and ZRS limb enhancer alleles each answer distinct questions. Ihh and Dhh overlap in gut and gonad, and Shh-Cre lines double as lineage drivers worth validating before use.
Shh knockin models place a defined sequence at the endogenous locus. Expression stays under native regulation, which matters for reporters, tags, and precise allele swaps.
What Shh Knockin mouse models are available?
ingenious targeting laboratory offers 2 distinct Shh knockin catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Shh Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Shh allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Shh Knockin mouse models are available?
When Shh Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Shh knockout embryonic lethal in mice?
It depends on background and allele design. Some Shh germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Shh animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Shh?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.