Sirpa Knockin mouse models
Encoding signal regulatory protein alpha (CD172a), Sirpa is the mouse ortholog of human SIRPA and presents three extracellular immunoglobulin domains, of which the polymorphic IgV domain binds CD47, plus a cytoplasmic tail with two ITIMs that recruit SHP-1 and SHP-2 to block phagocytic cup maturation and myosin IIA accumulation in macrophages, neutrophils, and dendritic cells. This CD47 to SIRPA axis is the dominant myeloid checkpoint that tumors exploit, and it is under strong species constraint because mouse SIRPA binds human CD47 poorly, which is exactly why humanized IgV knockin strains greatly improve human hematopoietic engraftment in immunodeficient hosts. Design forks sharply: conditional nulls or cytoplasmic truncations test ITIM-dependent inhibition in a defined myeloid lineage, whereas full ectodomain humanization is mandatory for anti-SIRPA or CD47 blocking antibodies and xenograft work. Related Sirpb1 and Sirpg do not restore inhibitory signaling, so target red cell clearance, antibody-dependent phagocytosis, or engraftment endpoints.
Targeting Sirpa preserves positional context compared with viral or BAC approaches. That helps when you quantify expression or map chromatin.
What Sirpa Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Sirpa knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Sirpa-Nod | Knockin | Immunodeficient mice | sperm cryopreservation | KI 232215 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Sirpa Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Sirpa allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Sirpa Knockin mouse models are available?
When Sirpa Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Sirpa knockout embryonic lethal in mice?
It depends on background and allele design. Some Sirpa germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Sirpa animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Sirpa?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.