Slc6a4 Knockout mouse models
Serotonin reuptake is executed by Slc6a4, the mouse ortholog of human SLC6A4, encoding SERT, an SLC6 family sodium and chloride dependent symporter with a twelve-transmembrane LeuT fold, a central substrate site, and an allosteric vestibular site, expressed on raphe serotonergic terminals as well as platelets, gut, and transiently on developing thalamocortical and sensory neurons. SERT is the direct target of SSRIs, MDMA, and cocaine, and it is regulated by PKC-dependent internalization, p38 MAPK phosphorylation, and syntaxin 1A interaction. Human variation includes the 5-HTTLPR promoter polymorphism and rare coding variants such as I425V. Slc6a4 knockout mice are viable with elevated extracellular serotonin, blunted 5-HT1A autoreceptor function, anxiety-like behavior, and abnormal barrel cortex patterning, making the null informative but developmentally confounded. Conditional alleles with tamoxifen-inducible Pet1-CreERT2 separate adult from developmental roles, and humanized alleles are preferred for SSRI pharmacology and promoter variant modeling.
Global Slc6a4 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Slc6a4 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Slc6a4 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Slc6a4-KO | Knockout | KO/CKO mice, disease model mice | live | KO 200892 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Slc6a4 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Slc6a4 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Slc6a4 Knockout mouse models are available?
When Slc6a4 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Slc6a4 knockout embryonic lethal in mice?
It depends on background and allele design. Some Slc6a4 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Slc6a4 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Slc6a4?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.