Smad1 Knockout mouse models
Bone morphogenetic protein signaling is transduced by Smad1, the mouse ortholog of SMAD1, a receptor-regulated SMAD whose MH1 domain binds GC-rich BMP response elements and whose MH2 domain is phosphorylated at a carboxy-terminal SSVS motif by the type I receptors ALK1, ALK2, ALK3 and ALK6, after which it trimerizes with SMAD4 and enters the nucleus. Signal duration is set in the linker region, where CDK8 and CDK9 prime and GSK3 extends phosphorylation to recruit the ubiquitin ligase SMURF1, terminating output. Germline deletion is embryonic lethal from allantois and chorioallantoic fusion failure, so conditional nulls with endothelial, chondrocyte or germ cell drivers are required for postnatal questions, and linker phospho-dead knockins uniquely test signal duration. Smad5 and Smad9 are genuinely redundant BMP effectors, so compound conditional alleles are usually necessary before concluding that a BMP-dependent process is Smad1 dependent.
Smad1 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Smad1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Smad1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Smad1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 251030 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Smad1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Smad1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Smad1 Knockout mouse models are available?
When Smad1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Smad1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Smad1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Smad1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Smad1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.