Smad4 Xenograft-Applicable mouse models
Every branch of the TGF beta superfamily converges on Smad4 (ortholog of human SMAD4), the sole common mediator SMAD, which trimerizes through its MH2 domain with two receptor-phosphorylated SMADs, either SMAD2 and SMAD3 downstream of TGF beta and activin or SMAD1, SMAD5 and SMAD9 downstream of BMP, and supplies nuclear export control plus the transcriptional activation surface for the complex. Because it is shared, deletion collapses both branches at once, which is informative for tumor suppression but confounds branch attribution. Germline loss is early embryonic lethal at gastrulation, and somatic inactivation defines juvenile polyposis and is a canonical event in pancreatic ductal adenocarcinoma, where conditional nulls combined with Kras G12D and Trp53 alleles reproduce the human progression sequence. When the question is branch-specific, receptor-SMAD conditional alleles are preferable to Smad4 deletion, and MH2 interface knockins test complex assembly without abolishing protein expression.
Orthotopic and subcutaneous routes both benefit from consistent Smad4 genetics across cages.
What Smad4 Xenograft-Applicable mouse models are available?
ingenious targeting laboratory offers 1 distinct Smad4 xenograft-applicable catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Smad4-Flox/Kras-LSL-G12D/Pdx1-Cre-Tg | Xenograft-Applicable | KO/CKO mice,point mutantion mice,Cre/Dre Toolbox of Mice, disease model mice, random transgenic mice | embryo cryopreservation | XA 234939 | Inquire |
Why this approach
We help teams pick alleles that match the experimental question, the organ system, and future breeding plans. For your target tissue centric work, Cre specificity, flox placement, and baseline controls matter as much as the gene edit itself.
Pricing and quotes
The Smad4 Xenograft-Applicable lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Smad4 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Smad4 Xenograft-Applicable mouse models are available?
When Smad4 Xenograft-Applicable lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Smad4 knockout embryonic lethal in mice?
It depends on background and allele design. Some Smad4 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Smad4 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Smad4?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.