Snca Knockout mouse models
Alpha-synuclein, encoded by mouse Snca and orthologous to human SNCA, is a 140 residue intrinsically disordered presynaptic protein with an amphipathic N-terminal region of imperfect KTKEGV repeats that folds into a helix on acidic membranes, a hydrophobic NAC segment driving amyloid assembly, and an acidic C-terminal tail mediating calcium and chaperone interactions. Physiologically it chaperones SNARE complex assembly through VAMP2 binding and modulates synaptic vesicle clustering; pathologically it forms Lewy body fibrils and propagates in a prion-like manner. Dominant missense alleles A53T, A30P, and E46K plus SNCA locus duplication and triplication cause Parkinson disease, establishing dosage as a driver. Critically, mouse Snca natively encodes threonine at position 53, so humanized knockin alleles are the correct choice for aggregation and antibody studies rather than relying on murine sequence. Redundancy with Sncb and Sncg is real, so pair conditional nulls with compound alleles when synaptic function is the endpoint.
Whole body loss of Snca gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Snca Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Snca knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Snca-KO | Knockout | KO/CKO mice, disease model mice | live | KO 190380 | Inquire |
Designing a Snca Knockout allele
A constitutive null is straightforward and unusually useful here, because a spontaneous deletion of this locus already exists in one widely used substrain, giving both a natural null and a standing caution about background genotype. Phenotypes are mild: modest reduction in striatal dopamine, altered vesicle recycling under sustained stimulation, and resistance to certain neurotoxins. The strongest applications are negative ones. Nulls resist pathology after inoculation with preformed fibrils, the definitive control establishing that seeding requires endogenous substrate, and the null serves as clean host for humanized or copy-number alleles. When designing the deletion, remove coding exons while leaving the immediately adjacent gene intact, since the spontaneous substrain deletion removes both and that confound has misled published work. Score voltammetric release, vesicle counts by electron microscopy, and fibril challenge resistance rather than baseline behavior.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Snca Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Snca allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Snca Knockout mouse models are available?
When Snca Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Snca knockout embryonic lethal in mice?
It depends on background and allele design. Some Snca germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Snca animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Snca?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.