Sost Knockout mouse models
Sost is the mouse ortholog of human SOST and encodes sclerostin, an osteocyte-derived, secreted cysteine-knot protein of the DAN family that binds the first beta-propeller of LRP5 and LRP6, using LRP4 as a presenting platform, to block Wnt, Frizzled, and LRP coreceptor assembly and suppress beta-catenin signaling in osteoblasts, with a secondary reported BMP-antagonist activity. Mechanical loading downregulates it, and human loss of function causes sclerosteosis or, through deletion of the ECR5 enhancer roughly 35 kilobases downstream, van Buchem disease, both high bone mass conditions that validated antibody blockade. This makes the allele fork unusually crisp: osteocyte-restricted conditional nulls using Dmp1-Cre test cell of origin and adult loading responses, ECR5 enhancer deletion models the human regulatory disease, and humanizing the LRP-binding loop enables direct antibody pharmacology. Sostdc1 shares the same LRP-binding mode, so consider compound alleles when dental or renal endpoints accompany skeletal readouts.
Global Sost deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Sost Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Sost knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Sost Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Sost allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Sost Knockout mouse models are available?
When Sost Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Sost knockout embryonic lethal in mice?
It depends on background and allele design. Some Sost germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Sost animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Sost?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.