Srebf1 Conditional Knockout mouse models
Srebf1, ortholog of human SREBF1, encodes membrane-bound basic helix-loop-helix leucine zipper transcription factors, SREBP-1a and the liver and adipose enriched SREBP-1c, produced from alternative promoters and first exons. Both are synthesized as endoplasmic reticulum precursors held by SCAP and, when sterols are abundant, retained through INSIG binding; sterol depletion permits COPII transport to the Golgi, sequential cleavage by S1P and S2P, and release of the nuclear form that occupies sterol response elements to drive Acaca, Fasn, Scd1 and Elovl6. SREBP-1c is additionally induced by insulin through AKT and by LXR agonism, making the locus the transcriptional hub of de novo lipogenesis. Hepatocyte or adipocyte conditional nulls address tissue-autonomous lipogenesis, while nuclear-form transgenes test sufficiency without processing control. Srebf2 partially substitutes at shared promoters, so measure both nuclear forms before attributing altered fatty acid synthesis to Srebf1 alone.
Tissue restricted knockout of Srebf1 keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells.
What Srebf1 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Srebf1 conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Srebf1-Flox | Conditional Knockout | KO/CKO mice | live | CKO 251664 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Srebf1 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Srebf1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Srebf1 Conditional Knockout mouse models are available?
When Srebf1 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Srebf1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Srebf1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Srebf1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Srebf1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.