Srsf2 Knockout mouse models
Srsf2 encodes the mouse ortholog of human SRSF2 (SC35), a serine and arginine rich splicing factor with a single RNA recognition motif and a phosphorylated RS domain that binds exonic splice enhancers, recruits U1 and U2 snRNP to nascent transcripts, and also couples to transcriptional elongation and R loop resolution. The recurrent P95H, P95L, and P95R substitutions in myelodysplastic syndrome, chronic myelomonocytic leukemia, and secondary acute myeloid leukemia are heterozygous and mutually exclusive with SF3B1 and U2AF1 lesions, acting by shifting exonic enhancer preference from GGNG to CCNG motifs, which drives poison exon inclusion in Ezh2 and aberrant splicing of Bcor rather than simple loss of activity. Model design therefore requires conditional heterozygous P95H knockins, since homozygous mutant and null alleles are not tolerated in hematopoietic cells, with Mx1-cre or Vav1-cre induction to capture dysplasia and clonal advantage. Align allele choice with endpoint, whether splicing catalogs, competitive transplantation, or PRMT5 and spliceosome inhibitor sensitivity.
Srsf2 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Srsf2 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Srsf2 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Srsf2-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 205052 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Srsf2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Srsf2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Srsf2 Knockout mouse models are available?
When Srsf2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Srsf2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Srsf2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Srsf2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Srsf2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.