Stat3 Conditional Knockout mouse models
Signaling from gp130-family cytokines, notably IL-6, IL-11, IL-10 and LIF, converges on Stat3, the ortholog of human STAT3, whose SH2 domain docks phosphorylated receptor tyrosines, is phosphorylated at Tyr705 by JAK1, dimerizes, and drives Socs3, Bcl3 and Myc transcription, with Ser727 phosphorylation and a separate mitochondrial pool supporting electron transport chain activity independently of transcription. Dominant-negative DNA-binding and SH2 domain mutations cause autosomal dominant hyper-IgE syndrome, whereas somatic activating mutations occur in large granular lymphocytic leukemia and germline gain-of-function alleles cause early-onset multiorgan autoimmunity, so patient-matched knockins interrogate mechanisms opposite in sign. Germline deletion is early embryonic lethal, making conditional nulls mandatory for essentially all adult work, and Ser727 phospho-dead knockins isolate the mitochondrial function. Match allele to endpoint: conditional null for tissue requirement, activating knockin for lymphoproliferation and JAK inhibitor response.
Tissue restricted knockout of Stat3 keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells.
What Stat3 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Stat3 conditional knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Designing a Stat3 Conditional Knockout allele
Choose the floxed interval so no fragment survives that can still dimerize. Flanking the exons encoding the SH2 domain prevents recruitment to receptor phosphotyrosines and avoids a truncated product that retains DNA binding and acts dominantly. Driver choice carries specific baggage. Lyz2-Cre deletion produces spontaneous chronic enterocolitis because myeloid cells lose interleukin 10 responsiveness, effectively phenocopying interleukin 10 deficiency and contaminating any other readout in that animal. Vil1-Cre impairs mucosal wound repair and worsens dextran sulfate sodium injury, Alb-Cre abolishes the hepatic acute phase response, Krt14-Cre disturbs hair cycling, and Cd4-Cre blocks Th17 differentiation. Because the protein is abundant and stable, confirm loss by phospho-Tyr705 flow cytometry after interleukin 6 or interleukin 10 stimulation rather than by genomic PCR, and use Socs3 induction as the functional readout.
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Stat3 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Stat3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Stat3 Conditional Knockout mouse models are available?
When Stat3 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Stat3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Stat3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Stat3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Stat3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.