Stat3 Knockout mouse models
Signaling from gp130-family cytokines, notably IL-6, IL-11, IL-10 and LIF, converges on Stat3, the ortholog of human STAT3, whose SH2 domain docks phosphorylated receptor tyrosines, is phosphorylated at Tyr705 by JAK1, dimerizes, and drives Socs3, Bcl3 and Myc transcription, with Ser727 phosphorylation and a separate mitochondrial pool supporting electron transport chain activity independently of transcription. Dominant-negative DNA-binding and SH2 domain mutations cause autosomal dominant hyper-IgE syndrome, whereas somatic activating mutations occur in large granular lymphocytic leukemia and germline gain-of-function alleles cause early-onset multiorgan autoimmunity, so patient-matched knockins interrogate mechanisms opposite in sign. Germline deletion is early embryonic lethal, making conditional nulls mandatory for essentially all adult work, and Ser727 phospho-dead knockins isolate the mitochondrial function. Match allele to endpoint: conditional null for tissue requirement, activating knockin for lymphoproliferation and JAK inhibitor response.
Global Stat3 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Stat3 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Stat3 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Stat3-KO(非移码) | Knockout | KO/CKO mice | live | KO 210186 | Inquire |
Designing a Stat3 Knockout allele
Germline loss kills the embryo shortly after implantation, before gastrulation completes, so the null exists to serve other alleles rather than to be phenotyped. Three legitimate uses remain. First, embryonic stem cell work, where the requirement for LIF driven self renewal makes the null informative in culture. Second, tetraploid complementation and chimera analysis for early lineage questions. Third, and most practical, placing the null in trans to a conditional allele so a single recombination event yields complete loss, which rescues experiments where a weak or mosaic driver leaves too many intact alleles. Do not use heterozygotes to model hyper-IgE syndrome; patient alleles act dominant negatively rather than by haploinsufficiency, so a heterozygote carries half the normal protein and none of the interfering species. Score embryo staging, epiblast morphology, and colony formation under defined stem cell conditions.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Stat3 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Stat3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Stat3 Knockout mouse models are available?
When Stat3 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Stat3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Stat3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Stat3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Stat3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.