Syngap1 Knockin mouse models
Synaptic Ras GTPase-activating protein, encoded by mouse Syngap1 and orthologous to human SYNGAP1, is among the most abundant postsynaptic density proteins, containing a pleckstrin homology domain, a C2 domain, a RasGAP catalytic domain active on both Ras and Rap, and a C-terminal PDZ-binding motif anchoring it to PSD-95. By restraining Ras-ERK signaling it limits AMPA receptor insertion and spine maturation, and its rapid NMDA receptor-driven phosphorylation and dispersal from the density is a required step in long-term potentiation. Alternative C-terminal isoforms alpha1, alpha2, beta, and gamma exert opposing effects on synaptic strength. Heterozygous de novo truncating variants cause SYNGAP1-related intellectual disability with epilepsy and autism, and homozygous null mice die within days of birth, so heterozygous or conditional alleles are mandatory. Choose conditional null for reversibility and cell-autonomy studies with excitatory or interneuron Cre drivers, isoform-specific alleles for the C-terminal question, and humanization for oligonucleotide upregulation testing.
Syngap1 knockin models place a defined sequence at the endogenous locus. Expression stays under native regulation, which matters for reporters, tags, and precise allele swaps.
What Syngap1 Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Syngap1 knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Syngap1-Linker-EGFP-3XFLAG | Knockin | fluorescent mouse | live | KI 252412 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Syngap1 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Syngap1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Syngap1 Knockin mouse models are available?
When Syngap1 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Syngap1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Syngap1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Syngap1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Syngap1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.