Tbk1 Knockout mouse models
Tbk1, the mouse ortholog of human TBK1, encodes a serine threonine kinase of the IKK family with kinase, ubiquitin-like, and scaffold dimerization domains that phosphorylates STING at Ser366 and IRF3 at Ser396 to drive type I interferon transcription downstream of cGAS, RIG-I through MAVS, and TLR3 through TRIF, and separately phosphorylates the autophagy receptors OPTN and SQSTM1 to license selective mitophagy and xenophagy. Human haploinsufficiency and loss of function mutations cause amyotrophic lateral sclerosis and frontotemporal dementia, while excess interferon output underlies its interest in autoinflammation. Germline nulls die at midgestation from TNF-dependent hepatic apoptosis, a lethality rescued on a Tnfrsf1a-null background, so practical alleles include kinase-dead knockins, phosphosite mutants, conditional deletion, and truncating patient variants. Ikbke partially covers interferon induction in myeloid cells, so compound genetics may be required, and allele choice should track interferon output, mitophagy flux, or motor neuron phenotypes.
Tbk1 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Tbk1 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Tbk1 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Tbk1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Tbk1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Tbk1 Knockout mouse models are available?
When Tbk1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Tbk1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Tbk1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Tbk1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Tbk1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.