Tet2 Knockin mouse models
Tet2 is the mouse ortholog of human TET2 and encodes an iron and 2-oxoglutarate dependent dioxygenase that oxidizes 5-methylcytosine to 5-hydroxymethylcytosine and further products, initiating active and passive demethylation at enhancers; unlike Tet1 and Tet3 it lacks a CXXC domain and instead relies on partners such as IDAX, WT1, and EBF1 for locus targeting. Truncating and catalytic domain missense mutations make TET2 one of the most frequent drivers of clonal hematopoiesis and of myelodysplastic syndrome, chronic myelomonocytic leukemia, and angioimmunoblastic T cell lymphoma, and its activity is phenocopied by IDH1 or IDH2 neomorphic mutations through 2-hydroxyglutarate inhibition while being stimulated by ascorbate. Germline nulls are viable with progressive hematopoietic stem cell expansion, myeloid bias, and long tumor latency, so conditional nulls with Vav1-cre or Mx1-cre plus a cooperating Flt3 internal tandem duplication or Jak2 V617F allele sharpen the phenotype. Paralogs Tet1 and Tet3 partially buffer hydroxymethylation, making compound conditional alleles worthwhile.
Tet2 knockin models place a defined sequence at the endogenous locus. Expression stays under native regulation, which matters for reporters, tags, and precise allele swaps.
What Tet2 Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Tet2 knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Tet2-HA | Knockin | otherToolbox of Mice(Non-Cre, fluorescent) | sperm cryopreservation | KI 241364 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Tet2 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Tet2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Tet2 Knockin mouse models are available?
When Tet2 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Tet2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Tet2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Tet2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Tet2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.